Fig. 4
From: Profiling the role of m6A effectors in the regulation of pluripotent reprogramming
IGF2BP1 knockdown altered pluripotency in H9 cells. H9 cells were transduced using two shRNAs (shIGF2BP1-2, eGFP, green). Pluripotency was assessed by immunostaining of markers SOX2 (A left, panel 3, red) and OCT4 (B left, panel 3, red) at 72-hour post-transduction. Scale bar, 20 μm. Quantification of the intensity of SOX2 (A, right panel) and OCT4 (B, right panel) fluorescence is represented as mean ± SEM (arbitrary units, N = 3)