Fig. 3
From: Profiling the role of m6A effectors in the regulation of pluripotent reprogramming
Knockdown of IGF2BP1 downregulates Stemness genes. A-B. Quantitation of stemness genes by real-time PCR in H9 and C11 cells. Cells were collected 48 h after three siIGF2BP1 treatments. β-Actin was used as the control. The decreased expression of IGF2BP1 and SOX2 transcripts was observed after transduction with IGF2BP1 siRNA in H9 and C11 cells. C. Left panel, Western blot of stemness genes. Cells were collected for Western blotting 72 h after siIGF2BP1 treatment in H9 and C11 cells. Right panel, quantification of the Western blot image using ImageJ software