Fig. 1
From: Profiling the role of m6A effectors in the regulation of pluripotent reprogramming
Identification of IGF2BP1 as an important pluripotency-associated m6A effector. A. Schematic diagram of the RNA-seq using human and mouse pluripotent stem cells and fibroblast. C11 iPSC: induced pluripotent stem cell line C11; H9 hESC: human embryonic stem cell line H9 (positive control); PSC: Pluripotent stem cells; SPF7: human skin fibroblasts. B Heatmaps depicting expression levels of m6A effector transcripts in human pluripotent stem cells (H9 and C11) and fibroblasts (SPF7). The expression levels were normalized by the row. C-E. Volcano plots of genes downregulated/upregulated in the indicated pairwise comparisons. Only genes with an average read count of three or greater across all samples are included. Genes with a log2-fold-change > 1or < − 1, and False Discovery Rate (FDR) < 0.05 are shown in red or blue (n = 1 for each cell line). Genes without significant difference (|log2-fold-change|<1 or FDR > 0.05) are shown in grey