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Table 1 Effect of single amino acid substitution on MAPK1 group I and II variants stabilitya

From: The complex impact of cancer-related missense mutations on the stability and on the biophysical and biochemical properties of MAPK1 and MAPK3 somatic variants

 

Tm (°C)

\(\Delta G^{{{\text{H}}_{{2}} {\text{O}}}}\) (kcal/mol)

m (kcal/mol/M)

[GdmCl]0.5 (M)

  

CD

([\(\Theta\)]222)

Fluorescence

(\(\overline{{\uplambda }}\))

CD

([\(\Theta\)]222)

Fluorescence

(\(\overline{{\uplambda }}\))

CD

([\(\Theta\)]222)

Fluorescence

(\(\overline{{\uplambda }}\))

NP-wild type

55.0

2.51 ± 0.22

2.79 ± 0.16

0.91 ± 0.09

1.34 ± 0.08

2.76

2.08

P-wild type

55.0

2.30 ± 0.16

2.88 ± 0.20

0.97 ± 0.07

1.19 ± 0.09

2.37

2.42

NP-E81K

55.0

1.10

9.03

2.76

7.02

1.77 ± 0.18

2.98 ± 0.40

2.07 ± 0.24

2.26 ± 0.70

0.62 ± 0.04

3.03 ± 0.08

1.33 ± 0.03

3.10 ± 0.08

P-E81K

54.1

6.04

5.37

5.23

4.72

4.91 ± 0.68

1.49 ± 0.37

4.91 ± 0.37

1.23 ± 0.30

1.23 ± 0.03

3.61 ± 0.14

1.07 ± 0.01

3.84 ± 0.26

NP-R135K

55.1

2.54

6.47

2.77 ± 0.25

2.28 ± 0.19

1.96 ± 0.51

1.32 ± 0.13

1.11 ± 0.02

3.30 ± 0.07

2.10

P-R135K

55.1

2.09 ± 0.17

2.38 ± 0.18

0.99 ± 0.07

1.18 ± 0.09

2.12

2.02

NP-D162G

49.0

60.0

2.28 ± 0.16

2.67 ± 0.12

1.05 ± 0.08

1.00 ± 0.05

2.17

2.68

P-D162G

60.0

3.45

2.53

2.11 ± 0.10

3.46 ± 0.58

1.10 ± 0.12

0.86 ± 0.05

1.00 ± 0.03

2.31 ± 0.05

2.45

NP-R191H

58.0

2.65

6.04

5.29 ± 1.06

2.00 ± 0.27

1.56 ± 0.41

2.76 ± 0.55

1.33 ± 0.04

3.87 ± 0.10

1.92

P-R191H

58.1

2.31 ± 0.19

2.55 ± 0.19

1.02 ± 0.09

1.35 ± 0.11

2.27

1.88

NP-Y316F

55.1

2.54 ± 0.23

2.78 ± 0.25

1.09 ± 0.10

1.39 ± 0.13

2.32

2.00

P-Y316F

55.1

1.46 ± 0.09

2.37 ± 0.19

0.93 ± 0.05

1.09 ± 0.09

1.57

2.17

NP-P319S

51.0

2.23

4.16

3.08

6.44

2.04 ± 0.27

1.17 ± 0.41

2.48 ± 0.23

1.88 ± 0.46

1.09 ± 0.03

3.56 ± 0.20

1.24 ± 0.02

3.43 ± 0.10

P-P319S

55.1

3.04 

4.30 

2.59 ± 0.24

2.79 ± 0.20

1.12 ± 0.34

1.29 ± 0.13

1.09 ± 0.01

3.84 ± 0.26

2.02

NP-E322V

57.1

4.10

4.14

2.60 ± 0.19

3.63 ± 0.65

1.19 ± 0.32

1.12 ± 0.09

1.13 ± 0.03

3.48 ± 0.15

2.31

P-E322V

56.0

1.89

5.43

2.60 ± 0.22

2.10 ± 0.28

1.60 ± 0.24

1.28 ± 0.11

0.90 ± 0.03

3.39 ± 0.07

2.04

  1. aMelting temperatures (Tm) were obtained as described in Materials and Methods by continuously monitoring the molar ellipticity at 222 nm every 0.5 °C. GdmCl-induced unfolding equilibrium data were measured as described in Materials and Methods by monitoring the ellipticity at 222 nm ([Θ222]) and the fluorescence intensity-averaged emission wavelength (λ¯¯¯λ¯, Eq. (2)). ΔGH2O and m values were obtained from Eq. (3); [GdmCl]0.5 was calculated from Eq. (5). [Θ222] and ‾λ data were fitted to Eq. (4) for the 2-state unfolding or to Eq. (6), for the 3-state unfolding. Data are reported as the mean ± SE of the fit. All the measures were performed on the non-phosphorylated (NP-) and phosphorylated (P-) form of the proteins
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Human Genomics

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