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Fig. 3 | Human Genomics

Fig. 3

From: The complex impact of cancer-related missense mutations on the stability and on the biophysical and biochemical properties of MAPK1 and MAPK3 somatic variants

Fig. 3

Spectral properties of non-phosphorylated (NP-) and phosphorylated (P-) MAPK1 (a, c, e) and MAPK3 (b, d, f) wild type. a, b Near-UV CD spectra were recorded in a 1.0-cm quartz cuvette at 1.3 mg/mL protein concentration in 20 mM Tris–HCl pH 7.5 containing 1.0 mM DTT and 0.1 M NaCl. c, d Intrinsic fluorescence emission spectra (295 nm excitation wavelength) were monitored at 130 µg/mL (0.08 AU 280 nm) in 20 mM Tris–HCl, pH 7.5, 0.1 M NaCl, 0.2 mM DTT. (E, F) Far-UV CD spectra were monitored in a 0.1-cm quartz cuvette at 130–170 µg/mL in 20 mM Tris–HCl, pH 7.5, 0.2 M NaCl and 0.2 mM DTT. The dashed lines are used for the NP-MAPK1 and N-PMAPK3; the continuous lines are used for P-MAPK1 and P-MAPK3. All spectra were recorded at 20 °C

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Human Genomics

ISSN: 1479-7364