Fig. 3
From: FGFR1 variants contributed to families with tooth agenesis
The variant FGFR1 (c.103G > A) promotes the expression of FGFR1 and proliferation, reduces apoptosis and affects the process of EMT in HEK293 and hDPSCs. a The fluorescence image shows the transfection efficiency of lentiviruses. b Protein and mRNA levels of FGFR1 were detected after transfection with FGFR1 overexpression lentivirus (control, wildtype and mutation). c Cell counting kit-8 assay was used to assess the proliferation after transfection with lentivirus (control, wildtype and mutation). d Quantitative analysis of cell apoptosis by flow cytometry between the three groups (control, wildtype and mutation). e Subcellular localization of wildtype or mutated FGFR1 in the HEK293 and hDPSCs. a'-f' Nuclei stanning by DAPI. b"-h" Merge of FGFR1 and DAPI. FGFR1 (red); nuclei (blue). Scale bars: 50 μm. f Protein levels of EMT markers E-cadherin, N-cadherin and Vimentin in the cells transfected by wildtype and mutation lentivirus were assayed by western blot