Fig. 4

Validation of ANXA1 expression level and correlation with prognosis. A Volcano map of the GSE179993 dataset, containing the transcriptome data of liver tissue samples from three 0.1% DDC-fed mice and three mice fed normal chow, and showing the expression of ANXA1. Volcano map of the GSE80776 dataset, containing the transcriptome data of liver tissue samples of five Mdr2^−/− and seven wild-type mice, and showing the expression of ANXA1. B Representative IHC and H&E staining images from serial liver sections in PSC and HC. C Quantification of ANXA1 expression in PSC and HC by analyzing the stained areas of IHC images. D The correlations between ANXA1 expression and risk score by using the revised natural history model for PSC, E and serum ALP. **p < 0.01. ANXA1, annexin A1; IHC, immunohistochemistry; H&E, hematoxylin and eosin staining; PSC, primary sclerosing cholangitis; HC, healthy control; ALP, alkaline phosphatase;