Fig. 1From: Generation and characterization of a zebrafish knockout model of abcb4, a homolog of the human multidrug efflux transporter P-glycoproteinGeneration of zebrafish abcb4 knockout mutant using CRISPER/Cas9 system a chematics of the abcb4 mutant alleles generated using CRISPER/Cas9. The 4th exon of abcb4 was targeted by gRNA. The sequences of the abcb4 wild-type (WT) and 2 nucleotides deletion mutant allele (abcb4−/−) were illustrated. Quantitative RT-PCR showed abcb4 transcript reduction in the embryos b and brain tissue c of the abcb4 mutant. Data are expressed as mean ± SD from at least three independent experiments. P-values were calculated using a two-tailed t-test or one-way ANOVABack to article page