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Fig. 4 | Human Genomics

Fig. 4

From: Transcriptome and proteome analysis reveals the anti-cancer properties of Hypnea musciformis marine macroalga extract in liver and intestinal cancer cells

Fig. 4

RT-qPCR and ChIP-qPCR analysis of selected target genes. A RT-qPCR analysis of known p53 target genes in HepG2 and Ls174 cells following treatment to seaweed extract. Control corresponds to untreated cells. Asterisks mark statistical significance based on ANOVA. B ChIP-qPCR for the HepG2-upregulated target genes, following immunoprecipitation with a-p53 in treated and untreated HepG2 and Ls174 cells. Y-axis corresponds to input normalized fold change compared to control loci. Asterisks mark statistical enrichment over control loci based on ANOVA. C ChIP-Western showing co-immunoprecipitation of the p300 protein with p53. D ChIP-qPCR for the HepG2 upregulated target genes, following immunoprecipitation with a-p300 in treated and untreated HepG2 and Ls174 cells. Y-axis corresponds to input normalized fold change compared to control loci. Asterisks mark statistical enrichment over control loci based on ANOVA

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Human Genomics

ISSN: 1479-7364