Table 1 Primer sequences for real-time PCR
Genes | 5′-3′ | Primers | Product Size (bp) | Reference sequence |
|---|---|---|---|---|
SM 22α | Forward Reverse | CCCGCCCTCCATGGTCTTCAAG GCCAAACTGCCCAAAGCCATTAC | 165 | NM_031549.1 |
Calponin | Forward Reverse | CGGGCACCAAGCGGCAGATCT CCGGGGTCAGGCAGTACTTGGGA | 165 | NM_031747.1 |
OPN | Forward Reverse | CATCAGAGCCACGAGTTTCA TCAGGGCCCAAAACACTATC | 274 | NM_012881.2 |
Runx2 | Forward Reverse | CAGACCAGCAGCACTCCATA CAGCGTCAACACCATCATTC | 178 | NM_001278483.1 |
GAPDH | Forward Reverse | AAGTTCAACGGCACAGTCAAGG CGCCAGTAGACTCCACGACATA | 139 | NM_017008.4 |