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Fig. 3 | Human Genomics

Fig. 3

From: EFTUD2 gene deficiency disrupts osteoblast maturation and inhibits chondrocyte differentiation via activation of the p53 signaling pathway

Fig. 3

eftud2 gene knockdown and knockout in zebrafish. a PCR analysis and gel electrophoresis to identify the efficacy of an eftud2 morpholino (EMO). On the left, amplification was performed with the EMO1 primer, which resulted in a clear band in WT zebrafish and nonspecific bands in EMO-injected larvae. On the right, amplification was performed with the EMO2 primer, which produced a clear band in EMO-injected zebrafish and nothing in the WT. b Phenotypes of mutant zebrafish subjected to EMO injection and control morpholino (mismatch morpholino, EMIS-MO)-injected zebrafish at 3 dpf. c Comparison of the numbers of curved larvae among zebrafish injected with EMO, EMO together with normal EFTUD2 mRNA (rescue group), or EMIS-MO. d Western blot analysis of Eftud2 protein levels between the WT (+/+) and homozygous (−/−) mutant zebrafish at 3 dpf. e DNA sequencing of WT (+/+), heterozygous (+/−), and homozygous (−/−) mutants at 3 dpf

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Human Genomics

ISSN: 1479-7364