Fig. 4From: Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencingmRNA abundance, protein expression level, and subcellular localization of TBX2 and TBX3. Plasmids were transfected into HEK 293T cells and harvested. a, d Relative mRNA expression of the blank vector, wild-type plasmid, and variants of TBX2 and TBX3 (n = 3). GAPDH was used as an internal control. b, e Western blot analysis of the blank vector, wild-type plasmid, and variants of TBX2 and TBX3. Actin was used as an internal control. c, f Density quantitation of TBX2 and TBX3 variant protein expression as shown in b and e (n = 3). g Western blot analysis of TBX3 variant protein degradation through the ubiquitin-proteasome pathway. MG-132 was the proteinase inhibitor and DMSO was used as control. h, i Representative images of immunofluorescence staining of TBX2 and TBX3 variants and wild-type proteins. *P < 0.05, **P < 0.01 versus WT; data represented here are obtained from three biological replicatesBack to article page