Table 4 The oligonucleotide PCR primers used for amplification of genomic DNA to obtain template
From: Multiplex SNaPshot—a new simple and efficient CYP2D6 and ADRB1 genotyping method
Multiplex pool | Fragment name | Sequence 5′ → 3′ | Length (bases) | Product size (bp) | SNV |
|---|---|---|---|---|---|
1 | P1PCR1 | agcccggtaacctgtcgt | 18 | 162 | rs1801252 |
ccatcagcagacccatgc | 18 | ||||
P1PCR2 | tggaggaggtcaggcttaca | 20 | 342 | rs16947 | |
ggtgcagaattggaggtcat | 20 | rs5030867 | |||
rs28371725 | |||||
P1PCR3 | gtgtggtggcattgaggact | 20 | 332 | rs72549346 | |
gtggggacgcatgtctgt | 18 | ||||
P1PCR4 | gatgcactggtccaaccttt | 20 | 223 | rs35742686 | |
ctggtgtaggtgctgaatgc | 20 | rs5030656 | |||
rs72549353 | |||||
rs72549351 | |||||
2 | P2PCR1 | gccttcaaccccatcatcta | 20 | 328 | rs1801253 |
ggccctacaccttggattc | 19 | ||||
P2PCR2 | ctcacctggtcgaagcagta | 20 | 145 | rs1065852 | |
ccatcttcctgctcctggt | 19 | rs5030862 | |||
P2PCR3 | cagctcggactacggtcatc | 20 | 272 | rs28371706 | |
cttgacaagaggccctgacc | 20 | ||||
P2PCR4 | gtcctttcccaaacccatct | 20 | 562 | rs3892097 | |
gtggggctaatgccttcat | 19 | rs5030655 | |||
rs5030865 | |||||
rs72549354 | |||||
rs72549356 | |||||
rs1135822 |